faecalis produces an alkaline butt, alkaline slant, no H2S, and no gas. flexneri produces an acid butt, an alkaline slant, no H2S, and no gas. vulgaris produces an acid butt, an acid or alkaline slant, H2S, and gas. coli produces an acid butt, an acid or alkaline slant, and no H2S, but does produce gas. All three from the eg are gram negative rods, facultative anaerobic.coli, Proteus vulgaris, and Shigella flexneri. Triple sugar iron (TRI) agar test to differentiate among the family of Enterobacteriaceae. ONPG negative in Gram positive and Gram negative, results:.Gram negative results: No acidity, no gas.Gram positive results: Acidity but not gas production.Salmonella cholerae-suis is a facultatively anaerobic gram-negative rod having both a respiratory and fermentative type of metabolism, it produces acid and sometimes gas.Alcaligenes faecalis is an obligately aerobic rod, coccal rod, or coccus that uses acetate, propionate, butyrate, and some other organic acids as a sole carbon and energy source because carbohydrates are not used, it does not produce acid or gas.coli is beta-Galactosidase positive since it can also use lactose as its sole source of carbon. coli produces acid and gas by catabolizing D-glucose (dextrose) and other carboxydrates. It will grow either aerobically or in the absence of oxygen, but better in its presence.ĭurham tube for fermentation activity and ONPG for beta-Galactosidase activity. coli, no green-sheen.ĭeep agar for cultivating anaerobic bacteria. Gram negative results 11-15-14 Growth but not E.Gram positive results 11-15-14 Little or non bacteria culture.Lactose nonfermenters such as Salmonella, Proteus, Pseudomonas form colorless to amber colonies.It contains the dyes eosin Y and methylene blue, which partially suppress the growth of gram-positive bacteria.coli will take up the dyes and form green to blue-black colonies with a metallic sheen Staphylococcus aureus will ferment the mannitol and form yellow zones in the reddish agar because phenol red becomes yellow in the presence of fermentation acids.7.5% sodium chloride inhibits the growth of most bacteria.Hemolytic bacteria produces clear zones around their colonies because of blood cell destruction.Gram negative results 11-15-14: Hemolytic bacteria (beta?).Gram positive results 11-15-14: No hemolytic bacteria.Streptococci and staphylococci are hemolytic.It distinguishes between hemolytic and non-hemolytic bacteria.coli produces white colonies Blood agar is selective and differential media. subtilis produces white to cram colonies E. Gram Negative: Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella arizonae, Shigella sonnei.subtilis, Enterococcus faecalis, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus. Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction.Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination.Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests.Proteins, Amino Acids, and Enzymes VIII: Urease Activity.Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence.Proteins, Amino Acids, and Enzymes VI: Oxidase Test and Bioluminescence.Proteins, Amino Acids, and Enzymes V: Coagulase and DNase Activity.Proteins, Amino Acids, and Enzymes IV: Catalase Activity.Proteins, Amino Acids, and Enzymes III: Gelatin Hydrolysis.Proteins, Amino Acids, and Enzymes II: Casein Hydrolysis.Proteins, Amino Acids, and Enzymes I: The IMViC Tests.Carbohydrates II: Triple Sugar Iron Agar Test.Carbohydrates I: Fermentation and β-Galactosidase Activity.The Steak-Plate Technique: Selective and Differential Media.
Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures.Sterilization and Microbiological Culture Media Preparation.Flagella Staining: West and Difco’s SpotTest Methods.Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin).Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedure.
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